06-114

Anti-Cathepsin D Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): cathepsin D, cathepsin D (lysosomal aspartyl protease), lysosomal aspartyl peptidase, lysosomal aspartyl protease
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_001900
• UniProt accession no.: P07339
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CTSD(1509)

Description

General description

Cathepsin D is synthesized as a 54kDa precursor which is proteolytically processed to an intermediate 48kDa single chain which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types, especially breast cancer. Cathepsin D is expressed in epithelial cells as well as in macrophages. Immunoreactivity of cancer cells and tissue infiltrating macrophages must be recorded separately.

Specificity

84% sequence homology to mouse and rat.

This antibody recognizes Cathepsin D.

Immunogen

Epitope: Heavy Chain

GST-tagged recombinant protein corresponding to human Cathepsin D at the heavy chain.

Application

Anti-Cathepsin D Antibody is an antibody against Cathepsin D for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Cathepsin D in A431 and HeLa cell lystes.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Cathepsin D on 10 µg of A431 cell lysate.

Target description

Observed molecular weight at ~58 kDa Protein is glycosylated and may run slightly higher than predicted.
Predicted molecular weight of the active form is ~46 kDa and ~ 54 kDa for the precursor form.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable