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05-817R-I

Sigma-Aldrich

Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant

clone 63-1C-8, from rabbit

Synonym(s):

H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

63-1C-8, monoclonal

species reactivity

human

technique(s)

dot blot: suitable
flow cytometry: suitable
western blot: suitable

isotype

IgGκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer10)

Gene Information

human ... HIST1H3F(8968)

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

Specificity

Recognizes Histone H3 phosphorylated at Serine 10.

Immunogen

Epitope: Phosphorylated Ser10
KLH-conjugated linear peptide corresponding to Human Histone H3 phosphorylated at Ser10.

Application

Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, is a recombinant rabbit monoclonal antibody that detects Histone H3 phosphorylated at serine 10. This purified recombinant mAb, also known as Anti-H3S10p, is published and validated in WB, Flow Cytometry, and is specificity verified by DB.
Dot Blot Specificity Analysis: AbSurance Histone Antibody Specificity Arrays (cat# 17-668) containing various unmodified and modified Histone peptides (see table) were probed with Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant 0.05ug/mL.

Flow Cytometry Analysis: 0.25 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in Jurkat T lymphocytes.


Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology

Quality

Evaluated by Western Blotting in colcemid treated HeLa cell lysate.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Histone H3 phosphorylated at serine 10 in 10 µg of colcemid treated HeLa cell lysate.

Target description

~ 17 kDa observed

Physical form

Format: Purified
Protein A purified
Purified rabbit monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zaleena Akheralie et al.
Development (Cambridge, England), 150(18) (2023-09-08)
The planarian Schmidtea mediterranea is a well-established model of adult regeneration, which is dependent on a large population of adult stem cells called neoblasts. Upon amputation, planarians undergo transcriptional wounding programs and coordinated stem cell proliferation to give rise to
Nicole Lindsay-Mosher et al.
PLoS genetics, 16(2), e1008613-e1008613 (2020-02-23)
The extracellular matrix (ECM) is important for maintaining the boundaries between tissues. This role is particularly critical in the stem cell niche, as pre-neoplastic or cancerous stem cells must pass these boundaries in order to invade into the surrounding tissue.
Regulation of chromatin structure by histone H3S10 phosphorylation.
Johansen, Kristen M and Johansen, J?rgen
Chromosome Research, 14, 393-404 (2006)
Casey Kimball et al.
Developmental biology, 457(1), 119-127 (2019-09-27)
Named for its assembly near exon-exon junctions during pre-mRNA splicing, the exon junction complex (EJC) regulates multiple aspects of RNA biochemistry, including export of spliced mRNAs from the nucleus and translation. Transcriptome analyses have revealed broad EJC occupancy of spliced
M Lucila Scimone et al.
Nature communications, 13(1), 2726-2726 (2022-05-19)
Regeneration often involves the formation of a blastema, an outgrowth or regenerative bud formed at the plane of injury where missing tissues are produced. The mechanisms that trigger blastema formation are therefore fundamental for regeneration. Here, we identify a gene

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