biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
78, monoclonal
mol wt
calculated mol wt 115.78 kDa
observed mol wt ~110 kDa
species reactivity
human, rat
packaging
antibody small pack of 100 μg
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
rat ... Ptk2b(50646)
General description
Protein-tyrosine kinase 2β (UniProt: P70600; also known as EC:2.7.10.2, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Pyk2) is encoded by the Ptk2b (also known as Pak2, Pyk2) gene (Gene ID: 50646) in rat. Pky2 is a non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. Its protein kinase domain is localized in amino acids 425-683. Pyk2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It is highly expressed in pulmonary vein endothelial cells, lung, and brain. It localizes to the cytoplasmic side of the membrane. Pyk2 is activated by phosphorylation of tyrosine residues in response to various stimuli that elevate intracellular calcium levels. Tyr402 is the major autophosphorylation site, but it can also be phosphorylated by other kinases. This phosphorylation promotes interaction with Src and Src family members, leading to phosphorylations at Tyr579, Tyr580, and Tyr881. Phosphorylation at Tyr402, Tyr579 and Tyr580 is required for its optimal kinase activity and phosphorylation at Tyr881 is essential for its interaction with Grb2.
Specificity
Clone 74 is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2β (Pyk2). It targets an epitope within the N-terminal half.
Immunogen
GST-tagged recombinant fragment corresponding to 412 amino acids from the N-terminal half of rat Protein-tyrosine kinase 2-beta (Pyk2).
Application
Quality Control Testing
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected Pyk2/CAKβ in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:250 dilution from a representative lot detected Pyk2/CAKβ in rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected Pyk2/CAKβ in Western Blotting applications (Bernard-Trifilo, J.A., et. al. (2005). J Neurochem. 93(4):834-49).
Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Pyk2/CAKβ in E18 rat cortex neurons.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected Pyk2/CAKβ in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:250 dilution from a representative lot detected Pyk2/CAKβ in rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected Pyk2/CAKβ in Western Blotting applications (Bernard-Trifilo, J.A., et. al. (2005). J Neurochem. 93(4):834-49).
Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Pyk2/CAKβ in E18 rat cortex neurons.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Pyk2/CAKβ, clone 74, Cat. No. 05-488-I, is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2b and is tested for use in Immunocytochemistry and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at +2°C to +8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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