04-894
Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W, rabbit monoclonal
culture supernatant, clone D1W, Upstate®
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
D1W, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pTyr1054)
Gene Information
human ... KDR(3791)
Specificity
KDR/Flk-1/VEGFR2 phosphorylated on Tyrosine 1054.
Predicted to have broad species cross-reactivity based on sequence homology.
Immunogen
KLH-conjugated, synthetic peptide encompassing the phosphorylated tyrosine residue 1054 of human KDR/Flk-1/VEGFR2.
Application
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
This Anti-phospho-KDR/Flk-1/VEGFR2 (Tyr1054) Antibody, clone D1W is validated for use in WB for the detection of phospho-KDR/Flk-1/VEGFR2 (Tyr1054).
Western Blot: 1:1000-1:4000 dilution for the detection of phospho-KDR/Flk-1/VEGFR2 in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
Quality
Routinely evaluated by immunoblot in RIPA lysates from CSF stimulated porcine aortic endothelial (PAE) cells expressing a human CSF-R1/murine KDR chimeric receptor.
Target description
152 kDa
Physical form
Cultured supernantant with 0.05% sodium azide
Storage and Stability
2 years at -20°C from date of shipment.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Gareth W Fearnley et al.
Biology open, 5(5), 571-583 (2016-04-06)
Vascular endothelial growth factor A (VEGF-A) binding to the receptor tyrosine kinase VEGFR2 triggers multiple signal transduction pathways, which regulate endothelial cell responses that control vascular development. Multiple isoforms of VEGF-A can elicit differential signal transduction and endothelial responses. However
Phosphorylation of Akt and ERK1/2 is required for VEGF-A/VEGFR2-induced proliferation and migration of lymphatic endothelium.
Dellinger, MT; Brekken, RA
Testing null
Edward J Hartsough et al.
Science signaling, 6(304), ra104-ra104 (2013-12-05)
Activation of vascular endothelial growth factor receptor-2 (VEGFR-2), an endothelial cell receptor tyrosine kinase, promotes tumor angiogenesis and ocular neovascularization. We report the methylation of VEGFR-2 at multiple Lys and Arg residues, including Lys(1041), a residue that is proximal to
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