Predicted cross-reactivity with human, rabbit, mouse and rat
Recognizes phosphorylated eNOS/NOS III.
Immunogen
Peptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III
Application
This Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
Western Blot Analysis: A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
Quality
routinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate
Target description
135 kDa
Linkage
Replaces: 05-811
Physical form
Cultured supernatant in 0.05% sodium azide
Analysis Note
Control Fetal bovine heart endothelial cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 55, e11612-e11612 (2022-02-10)
Anoikis is a type of apoptosis that occurs in response to the loss of adhesion to the extracellular matrix (ECM). Anoikis resistance is a critical mechanism in cancer and contributes to tumor metastasis. Nitric oxide (NO) is frequently upregulated in
The Journal of pharmacology and experimental therapeutics, 351(3), 500-509 (2014-09-23)
At the cardiovascular level, nitric oxide (NO) controls smooth muscle functions, maintains vascular integrity, and exerts an antihypertensive effect. Metal-nonoates are a recently discovered class of NO donors, with NO release modulated through the complexation of the N-aminoethylpiperazine N-diazeniumdiolate ligand
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