04-1542

Anti-ETS1 DBD Antibody, clone 1ET 7F2

ascites fluid, clone 1ET 7F2, from mouse

• Synonym(s): Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1, ets protein, v-ets avian erythroblastosis virus E2 oncogene homolog 1, v-ets avian erythroblastosis virus E26 oncogene homolog 1, v-ets erythroblastosis virus E26 oncogene homolog 1, v-ets eryt
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1ET 7F2, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_005229.1
• UniProt accession no.: P14921
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ETS1(2113)

Description

General description

ETS1 belongs to a family of transcription factors that regulates expression of several genes participating in extracellular matrix remodeling. Members of the ETS family regulate a variety of genes involved in stem cell development, cell senescence, and tumorigenesis. ETS1 is typically absent in healthy gastric epithelium, but expressed in many gastric carcinomas and oral squamus cell carcinomas.
This family of transcription factors contains 2 main domains; a transactivation domain (TA), and a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes. This DNA-Binding-Domain is highly conserved among ETS family members.

Specificity

This antibody recognizes ETS1.

Immunogen

Epitope: Unknown

Linear peptide corresponding to human ETS1 DBD.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-ETS1 DBD Antibody, clone 1ET 7F2 (Mouse Monoclonal Antibody) validated in WB to detect ETS1 DBD also known as Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1, ets protein.

Quality

Evaluated by Western Blot in HL60 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected ETS1 DBD on 10 µg of HL60 cell lysate.

Target description

~ 50 kDa

Physical form

Unpurified

Unpurified mouse monoclonal IgG1κ in preservative-free ascites.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HL60 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable