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03-307

Sigma-Aldrich

Magna ChIRP® Negative Control Probe Set

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
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product line

Magna ChIRP®

Quality Level

technique(s)

DNA sequencing: suitable
bead-based assay: suitable (purification with magnetic beads)
nucleic acid detection: suitable (RNA)
protein quantification: suitable

General description

ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target for biotinylated antisense probes. The Magna ChIRP Negative Control Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the mRNA sequence of Escherichia coli lacZ (?-galactosidase). All probes are biotin-TEG-labeled at the 3-prime end. Because LacZ gene is normally absent from mammalian cells, this primer set is ideal for use as negative control when performing ChIRP experiments with mammalian cells. This probe set is fully compatible with the MAGNA CHIRP® kits (cat. # 17-10494 and 17-10495).

Application

The Magna ChIRP Negative Control Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the mRNA sequence of lacZ (Β-galactosidase) and is used as a negative control for ChIRP experiments.

Other Notes

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Legal Information

MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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