03-209

SET7/9 human recombinant - Methyltransferase enzyme

Human recombinant SET7/9 expressed in E. Coli.

• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• assay: >95% (SDS-PAGE)
• mol wt: ~41 kDa
• packaging: pkg of 100 μg
• manufacturer/tradename: Upstate^®
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 1 mg/mL
• technique(s): enzyme immunoassay: suitable
• UniProt accession no.: Q8WTS6

Description

General description

The methylation of histones plays a pivotal role in the regulation of chromatin structure and gene expression. Histone methylation can occur on Arg or Lys residues, with an exquisite site selectivity for Lys methylation at specific positions in the N-termini of histones H3 and H4. SET7/9, a histone methyltransferase (HMTase), which transfers methyl groups to Lys4 of Histone H3, forms a complex with S-adenosyl-L-methionine. This complex contains an active site consisting of a binding pocket where an AdoMet molecule, in an unusual conformation, binds a narrow substrate-specific channel that only unmethylated lysine residues can access, and a catalytic tyrosine residue.

Specificity

<1.0EU/µg protein (LAL test)

Application

Human recombinant SET7/9 expressed in E. Coli.

Physical form

Format: Purified

In liquid containing 50 mM Tris-HCl, pH 7.5, containing 0.2 M sodium chloride, 5 mM DTT and 20% glycerol. Frozen solution.

Storage and Stability

Stable for 12 months at -20°C from date of receipt. Avoid multiple freeze/thaw cycles.

Analysis Note

Methyltransferase assays

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1