03-0182-00

SMC^® Human IL-12p70 High Sensitivity Immunoassay Kit

• Synonym(s): IL-12p70 Single Molecule Counting Kit, IL-12p70 ultra-sensitive immunoassay
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• species reactivity: canine, rat, mouse, feline, monkey
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.1 pg/mL; (Lower Limit of Quantification); inter-assay cv: <20%; intra-assay cv: <15%; (Inter-assay n=5; Intra-assay n=20)
• technique(s): single molecule counting: suitable
• storage temp.: 2-8°C

Description

Specificity

Species Reactivity:
Although IL-12p70 was detectable in rat, mouse, monkey, canine, and feline plasma, the assay has not been validated for these species.

Cross Reactivity:
Cross-reactivity to interferons of other types was tested using SMC^® Human IL-12p70 High Sensitivity Immunoasay Kit with the following results:

• rhIL-12p40 was found to be < 2.0% cross-reactive
• rhIL-23 was found to be < 1.5% cross-reactive
• rhILp35 was not found to be cross-reactive

Accuracy

• Minimum of 5 serum samples spiked with average recovery of 103% with a range of 100-106%
• Minimum of 5 plasma samples spiked with average recovery of 97% with a range of 84-102%

Application

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human IL-12p70 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-12p70 in human serum and plasma.

This new streamline kit has been improved with the following features over the legacy IL-12 kit, #03-0057-00:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna
• Matched to WHO standard

Research Category:
Inflammation & Immunology

Research Sub-Category:

• Inflammatory Disease
• Immune Response
• Autoimmune disease

Packaging

96 well assay

Components

• Assay Buffer
• IL-12p70 Coated Beads
• Standard Diluent
• IL-12p70 Detection Antibody
• IL-12p70 Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H412
• pcodes: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects