S1402

Sulforhodamine B sodium salt

powder, BioReagent, suitable for cell culture

• Synonym(s): Kiton Red 620
• Empirical Formula (Hill Notation): C₂₇H₂₉N₂NaO₇S₂
• CAS Number: 3520-42-1
• Molecular Weight: 580.65
• Colour Index Number: 45100
• Beilstein/REAXYS Number: 3886008
• EC Number: 222-529-8
• MDL number: MFCD00010180
• UNSPSC Code: 12352207
• PubChem Substance ID: 24899477
• NACRES: NA.75

Properties

• biological source: synthetic (organic)
• product line: BioReagent
• form: powder
• composition: Dye content, ~75%
• technique(s): cell culture | mammalian: suitable
• solubility: methanol: 1 mg/mL
• fluorescence: λ_ex 565 nm; λ_em 586 nm in H₂O
• SMILES string: [Na+].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S([O-])(=O)=O)=[N+](\CC)CC)c1
• InChI: 1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
• InChI key: SXQCTESRRZBPHJ-UHFFFAOYSA-M

Description

General description

Sulforhodamine B is a rhodamine derived aminoxanthene dye. It contains two sulfonic acids groups which separate in alkaline condition.

Application

Sulforhodamine B sodium salt has been used:

• for relative total cell mass quantification
• to investigate the viscosity of coacervates
• as a molecular rotor for microviscosity determination in aerosol droplets

Biochem/physiol Actions

Sulforhodamine B sodium salt is useful to measure cell biomass and also for cytotoxicity screening.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)