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P3889

Sigma-Aldrich

Polygalacturonic acid

≥85% (titration), from oranges

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About This Item

Linear Formula:
(C6H8O6)n
CAS Number:
MDL number:
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

oranges

assay

≥85% (titration)

form

powder

color

white to brown

mp

60 °C

solubility

aqueous NaOH: 1%, clear to hazy

storage temp.

room temp

InChI

1S/C6H10O7/c7-1-2(8)4(5(10)11)13-6(12)3(1)9/h1-4,6-9,12H,(H,10,11)/t1-,2+,3+,4-,6-/m0/s1

InChI key

AEMOLEFTQBMNLQ-BKBMJHBISA-N

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General description

Polygalacturonic acid is composed of galacturonic acid unit arranged as a long polymer chain. Methyl esters of polygalacturonic acid forms pectin.

Application

Polygalacturonic acid has been used:
  • in the preabsorption of 2F4 primary antibody solution
  • as a substrate to determine polygalacturonase activity
  • in the preparation of oligogalacturonates mixture which is used as a substrate to determine the specificities of different acetylesterases

Polygalacturonic acid sequesters metal ions. It also produces gels in the presence of Ca2+ that can be used for drug release or to immobilize cells or enzymes for the production of biomolecules.

Biochem/physiol Actions

In land plants, polygalacturonic acids are major components of cell wall polysaccharides (pectins). Bound polygalacturonase-inhibiting peptides (PGIP) protect pectin from degradation by fungal polygalacturonases.

Other Notes

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pectin acetylesterases from Aspergillus are able to deacetylate homogalacturonan as well as rhamnogalacturonan
Bonnin E, et al.
Carbohydrate Polymers, 74(3), 411-418 (2008)
Pectin acetylesterases from Aspergillus are able to deacetylate homogalacturonan as well as rhamnogalacturonan
E. Bonnin.
Carbohydrate Polymers, 74, 411-418 (2008)
Kun Cheng et al.
Drug development and industrial pharmacy, 30(4), 359-367 (2004-05-11)
Insulin-loaded calcium pectinate nanoparticles were prepared as a potential colonic delivery system by ionotropic gelation with calcium ions. The effects of pectin molecular weight (Mv) and formulation pH on the characteristics of the nanoparticles were evaluated. Commercial pectins, LM101 and
Marianne Hiorth et al.
International journal of pharmaceutics, 308(1-2), 25-32 (2005-12-06)
This study has investigated the potential of immersion coating calcium containing pellet cores first with pectin, and then with two different cross-linkers, calcium or chitosan. The interaction between pectin and calcium, and between pectin and chitosan, are believed to slow
Zhangyong Dong et al.
BMC biochemistry, 12, 51-51 (2011-09-17)
Fusarium wilt is an economically devastating disease that affects banana production. Although Cavendish banana cultivars are resistant to Fusarium oxysporum f.sp. cubense race 1 (FOC1) and maitain banana production after Gros Michel was destructed by race 1, a new race

Protocols

To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.

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