DFF100
DEAE–Sepharose™
Fast Flow
Synonym(s):
Diethylaminoethyl–Sepharose™
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
Quality Level
form
suspension
technique(s)
affinity chromatography: suitable
matrix
6% cross-linked agarose
bead size
45-165 μm (wet)
pore size
~4,000,000 Da exclusion limit
pH
2—12
capacity
110-160 μeq/mL binding capacity(gel volume)
Looking for similar products? Visit Product Comparison Guide
Related Categories
General description
DFF100-500Ml′s update product number is GE17-0709-01
Application
DEAE-Sepharose has been used in:
- anion exchange chromatography
- to screen polyisoprenyl phosphate phosphatase activity
- to purify isoinhibitors
- for the purification of human immunodeficiency virus (HIV)
- glycoprotein envelope (gp140 env)
DEAE-Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.
Biochem/physiol Actions
Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.
Legal Information
Sepharose is a trademark of Cytiva
Replaced by
Product No.
Description
Pricing
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk_germany
WGK 1
flash_point_f
100.4 - 109.4 °F
flash_point_c
38 - 43 °C
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
G L Yewey et al.
Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)
Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal
Jessie Fernandez et al.
mBio, 12(1) (2021-02-11)
Rice blast disease caused by Magnaporthe oryzae is a devastating disease of cultivated rice worldwide. Infections by this fungus lead to a significant reduction in rice yields and threats to food security. To gain better insight into growth and cell
Advances in Potato Chemistry and Technology (2016)
Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems
Rosenberg Y, et al.
Testing, 8(3), e58724-e58724 (2013)
Effective Purification Procedure of <I>Aspergillus oryzae</I> α−Amylase from Solid State Fermentation Cultures Including Concanavalin-A Sepharose
Terebiznik, M.R., et al.
Journal of Food Biochemistry, 19(5), 341-354 (1995)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service