C0858
Cyclooxygenase 2 human
≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein
Synonym(s):
COX-2, Prostaglandin H synthase 2
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About This Item
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recombinant
expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.
expressed in insect cells
Quality Level
assay
≥70% (SDS-PAGE)
form
aqueous solution
specific activity
≥8000 units/mg protein
mol wt
dimer subunit mol wt 70-74 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PTGS2(5743)
Biochem/physiol Actions
COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.
Other Notes
Tagged with a six-residue histidine sequence near the N-terminus.
Unit Definition
One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.
Physical form
Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol
Substrates
KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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