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C0733

Sigma-Aldrich

Cyclooxygenase 1 from sheep

glycerol solution, ≥1500 units/mg protein

Synonym(s):

COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase

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5000 UNITS
$927.00

$927.00

List Price$1,030.00Save 10%

Estimated to ship onMay 31, 2025

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5000 UNITS
$927.00

About This Item

Enzyme Commission number:
1.14.99.1 (BRENDA, IUBMB)
MDL number:
MFCD02098124
UNSPSC Code:
12352204
NACRES:
NA.32

$927.00

List Price$1,030.00Save 10%

Estimated to ship onMay 31, 2025

form

glycerol solution

Quality Level

200

specific activity

≥1500 units/mg protein

mol wt

dimer subunit mol wt 70 kDa

UniProt accession no.

O78749

shipped in

dry ice

Storage temp.

−70°C

Gene Information

sheep ... COX1(808251)

General description

Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain.[1] Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.[2]

Application

Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples[3] and in prostaglandin synthase activity assay.[4]

Biochem/physiol Actions

COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent KM of 8.3 μM for arachidonic acid.

Unit Definition

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

Physical form

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN® 20, 300 μM diethyldithiocarbamate and 10% glycerol.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000376044
0000366054
0000366054
0000376044
SLCN3278

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S Ohki et al.
The Journal of biological chemistry, 254(3), 829-836 (1979-02-10)
The highly purified prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes had two still unresolved enzyme activities; the oxygenative cyclization of 8,11,14-eicosatrienoic acid to produce prostaglandin G1 and the conversion of the 15-hydro-peroxide of prostaglandin G1 to a 15-hydroxyl group
Cyclo-oxygenase isoenzymes: physiological and pharmacological role
Kam PCA and See AUL
Anaesthesia, 55(5), 442-449 (2000)
Maurizio Anzini et al.
Journal of medicinal chemistry, 56(8), 3191-3206 (2013-03-29)
A series of 3-substituted 1,5-diarylpyrroles bearing a nitrooxyalkyl side chain linked to different spacers were designed. New classes of pyrrole-derived nitrooxyalkyl inverse esters, carbonates, and ethers (7-10) as COX-2 selective inhibitors and NO donors were synthesized and are herein reported.
The Sigma class glutathione transferase from the liver fluke Fasciola hepatica
LaCourse EJ, et al.
PLoS Neglected Tropical Diseases, 6(5), e1666-e1666 (2012)
Michela Zago et al.
Toxicology letters, 226(2), 107-116 (2014-01-30)
Diseases due to cigarette smoke exposure, including chronic obstructive pulmonary disease (COPD) and lung cancer, are associated with chronic inflammation typified by the increased expression of cyclooxygenase-2 (COX-2) protein. RelB is an NF-κB family member that suppresses cigarette smoke induction
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