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28713

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LB Broth

NutriSelect® Plus, from non-animal source(Vegitone), powder, pkg of 500 g, non-sterile, nonselective for Escherichia coli

Synonym(s):

E. coli enrichment broth, LB Broth, Vegitone, Luria Bertani Broth, Vegitone, Vegitone LB Broth

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500 G
$116.00

$116.00

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500 G
$116.00

About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

$116.00

In StockDetails

Request a Bulk Order

biological source

non-animal source (Vegitone)

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

sodium chloride, 5.0 g/L
tryptone (vegetable), 10.0 g/L
yeast extract, 5.0 g/L

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

color

clear to slightly opaque yellow to amber Prepared media
light yellow Dehydrated powder

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
food and beverages
life science and biopharma
microbiology
pharmaceutical

suitability

nonselective for Escherichia coli (Recombinant)
nonselective for coliforms

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General description

Luria Bertani Broth Vegitone is free of animal derived material. It is used for the cultivation and maintenance of recombinant strains of Escherichia coli and may be used for routine cultivation of not particularly fastidious microorganisms.

Preparation Note

Suspend 20 g in 1000 ml distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Dispense as desired.

Storage and Stability

  • Store prepared media below 8°C, protected from direct light.
  • Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM1908
BCCL1559
BCCK3270
BCCH8640
BCCG5978

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Questions

  1. Sir please confirm lb broth is equilant to lethene broth

    1 answer

    1. The formulations for LB Broth and Letheen Broth are quite different. LB Broth is typically used for the propagation of E. coli bacteria for molecular biology applications. Letheen Broth is typically used to demonstrate the efficacy of preservatives. Please see the formulations of each item below:
      LB Broth, 28713:
      Yeast Extract – 5.0 g/L
      Peptone, from casein – 10.0 g/L
      Sodium Chloride – 10.0 g/L

      Letheen Broth, 1.10405
      Peptone, from meat – 20.0 g/L
      Peptone, from casein – 5.0 g/L
      Meat extract – 5.0 g/L
      Yeast extract – 2.0 g/L
      Sodium Chloride – 5.0 g/L
      Lecithin – 0.7 g/L
      Sodium Bisulfite – 0.1 g/L

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