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LH20100

Supelco

Sephadex® LH-20 Size Exclusion Resin

140-550 mesh

Synonym(s):

Lipophilic Sephadex®

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About This Item

UNSPSC Code:
13111000
NACRES:
NB.21

product name

Sephadex® LH-20,

form

slurry

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

swelling

1 g swells to 4 mL (water, methanol)

bead size

25-100 μm

operating pH range

2-13

separation technique

size exclusion (SEC)

storage temp.

room temp

Related Categories

Application

Sephadex® LH-20 is a crosslinked dextran-based resin used with organic solvent mobile phases for gel permeation, normal phase partition, and adsorption chromatography of lipids, steroids, fatty acids, hormones, vitamins, etc. It is also used in gel filtration chromatography, protein chromatography, gel filtration media, resins and separation media.
Sephadex® LH-20 has been used to isolate bioactive polyphenolic content from blackberry genotypes and tryptophan from the aqueous extract of lotus rhizomes.

Legal Information

Sephadex is a registered trademark of Cytiva

replaced by

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S K Kundu et al.
Journal of lipid research, 20(7), 825-833 (1979-09-01)
Aminopropyl silica gel was prepared from porous silica gel and was used as a solid support for immunoadsorbent in the purification of anti-glycolipid antibodies. For neutral glycosphingolipids, a carboxyl function was generated by oxidation of the olefinic double bond of
Yizhong Cai et al.
Journal of chromatographic science, 43(9), 454-460 (2005-10-11)
HPLC characterization of reversed-phase (RP) high-performance liquid chromatography (HPLC) has been widely used in separation and identification of plant pigments. An effective RP-HPLC-based method is established to systematically isolate, identify, and quantitate the betalain pigments in the plants of 37
Suman B Sharma et al.
Journal of medicinal food, 14(4), 353-359 (2011-03-05)
Diabetes is accompanied by lipid abnormalities, which contribute significantly to cardiovascular morbidity and mortality in diabetic patients. We previously demonstrated the potent antihyperglycemic activity of the active principle (fraction II from Sephadex LH 20 chromatography [LH II]) isolated from ethanolic
Edith O Cuevas-Rodríguez et al.
Journal of agricultural and food chemistry, 58(12), 7458-7464 (2010-05-29)
This study was designed to characterize and compare wild, commercial, and noncommercial cultivated blackberry genotypes grown in Michoacan, Mexico. Six genotypes, including WB-3, WB-7, WB-10, and WB-11 (all wild blackberry types), Tupy (a commercial cultivar), and UM-601 (a cultivated breeding
A K Tandon et al.
Cancer research, 46(7), 3375-3377 (1986-07-01)
We described recently a modified charcoal-gelatin (MCG) assay for measuring progesterone receptor activity in low-protein cytosols. We showed that pre-mixing of gelatin with sample cytosols (final gelatin concentration 0.1%) and removal of unbound steroid by a 1% charcoal suspension with
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Protocols

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Column Packing & Separation with Sephadex® LH-20

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

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