GE17-0090-01
Sephadex® LH-20
Cytiva 17-0090-01, pack of 100 g
Synonym(s):
Sephadex Gel
About This Item
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feature
autoclavable 20 min at 121°C
packaging
pack of 100 g
manufacturer/tradename
Cytiva 17-0090-01
matrix
hydroxypropylated, cross-linked dextran
particle size
163 μm (in methanol max.)
18-111 μm (dry)
27 μm (In Methanol Min.)
cleaning in place
2-13
working range
2-13
Related Categories
General description
Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.
Sephadex® LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex® LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility
Features and Benefits
- Designed for use in polar organic solvents and aqueous solvent mixtures
- Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
- Useful for both analytical and industrial scales for the preparation of closely related molecular species
- Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
- Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
- Excellent batch-to-batch reproducibility
- Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
Storage and Stability
Analysis Note
Legal Information
Storage Class Code
13 - Non Combustible Solids
Certificates of Analysis (COA)
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Protocols
Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.
Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.
Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.
Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.
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