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DNTP100A

Sigma-Aldrich

Deoxynucleotide Set, 100 mM

Individual dNTPs for routine PCR; 1 mL each

Synonym(s):

PCR dNTPs, dNTP set, dNTPs

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About This Item

UNSPSC Code:
41106305
NACRES:
NA.52

Quality Level

form

liquid

concentration

100 mM

color

colorless

shipped in

dry ice

storage temp.

−20°C

General description

Deoxynucleotide triphosphates (dNTPs) are negatively charged organic molecules, which contains nucleobase bound to deoxyribose. This dNTPs set includes 1.0 mL each of 100mM aqueous solutions of four deoxyribonucleotides namely deoxyribose adenosine triphosphate (dATP), deoxyribose cytidine triphosphate (dCTP), deoxyribose guanosine triphosphate (dGTP), and deoxyribose thymidine triphosphate (dTTP) in separate tubes for convenience and easy PCR reaction setup.

Application

Deoxynucleotide set, 100 mM has been used in:
  • the amplification of DNA in polymerase chain reaction (PCR)
  • quantitative PCR (qPCR)
  • multiplex PCR

Biochem/physiol Actions

Deoxynucleotide triphosphates (dNTPs) are building blocks of nucleic acid molecules and are acidic in nature. They act as substrates for DNA polymerizing enzymes. dNTPs are essential components of PCR master mixes as it is necessary for DNA amplification reaction. Optimal levels of dNTPs ensure proper chromosomal replication and in turn maintain genome stability.

Features and Benefits

  • Provided as 4 separate tubes of dNTPs
  • Allows optimization of individual dNTP concentrations

Packaging

1.0 mL each of 100 mM dATP, dCTP, dGTP and dTTP

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oocyte source and hormonal stimulation for in vitro fertilization using sexed spermatozoa in cattle
Presicce GA, et al.
Veterinary Medicine International, 2011 (2010)
Characterization and Antimicrobial-Resistance Profile of Escherichia coli O157 and O157: H7 Isolated from Modified Atmosphere Packaged Meat Samples
Cad?rc?, et al.
Turkish Journal of Agriculture - Food Science and Technology null
A critical balance: dNTPs and the maintenance of genome stability
Pai CC and Kearsey SE
Gene, 8(2), 57-57 (2017)
Aleksandr M Agafontsev et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 25(11), 2684-2694 (2018-10-06)
Nucleotides are constituents of nucleic acids and they have a variety of functions in cellular metabolism. Synthetic receptors and sensors are required to reveal the role of nucleotides in living organisms and mechanisms of signal transduction events. In recent years
Establishment of a Fusarium graminearum Infection Model in Arabidopsis thaliana Leaves and Floral Tissues
Nalam, et al.
Bio-protocol, 6 (2016)

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