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71004-M

Sigma-Aldrich

10mM dNTP Mix

Qualified for enzymatic DNA synthesis

Synonym(s):

10mM dNTP mix, Deoxynucleoside triphosphate mix 10 mM solution, dNTP mix, dNTPs

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About This Item

UNSPSC Code:
41106301
NACRES:
NA.55

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

DNA synthesis: suitable

storage temp.

−20°C

General description

Deoxynucleoside triphosphate (dNTP) acts as a substrate for DNA polymerase. It helps in the synthesis of copies of the target DNA by providing each of the four bases (dATP, dGTP, dCTP, dTTP).

The10 mM dNTP Mix is a ready-to-use preparation of ultrapure dATP, dCTP, dGTP, anddTTP (monosodium salts) at a concentration of 10 mM each in sterile deionizedwater at pH 7.0.

Application

10mM dNTP Mix is suitable for synthesizing complementary DNA (cDNA) from RNA extracted from osteosarcoma cell lines.

Features and Benefits

  • The dNTP Mix is free of RNase and DNase
  • Qualified for any application that requires pure deoxynucleotides, such as (polymerase chain reaction) PCR, cDNA synthesis, and fill-in reactions

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Chapter 8 - DNA Sequencing
Clark D P, et al.
Molecular Biology and Physiology of Insulin and Insulin-Like Growth Factors, 240-269 (2019)
Huan Wang et al.
Molecular medicine reports, 16(4), 5157-5164 (2017-08-30)
Osteosarcoma is among the most malignant types of tumor worldwide and has become a leading contributor to tumor incidence, particularly in adolescents. Resistance to conventional treatment and the complexity of osteosarcoma tumorigenesis has resulted in high mortality rates. MicroRNAs are

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