SRP0364
mTOR/Raptor/MLST8 human
recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥50% (SDS-PAGE)
form
aqueous solution
mol wt
137 kDa (mTOR)
150 kDa (Raptor)
37 kDa (MLST8)
packaging
pkg of 10 μg
manufacturer/tradename
Sigma-Aldrich
technique(s)
cell based assay: suitable
solubility
water: soluble
NCBI accession no.
application(s)
life science and biopharma
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MLST8(64223) , MTOR(2475) , RPTOR(57521)
General description
This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.
Application
Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.
Biochem/physiol Actions
Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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mTOR controls cell cycle progression through its cell growth effectors S6K1 and 4E-BP1/eukaryotic translation initiation factor 4E.
Fingar DC
Molecular and Cellular Biology, 24(1), 200-216 (2004)
Belen Rubio-Viqueira et al.
Advances in experimental medicine and biology, 587, 309-327 (2006-12-14)
The mammalian target of rapamycin (mTOR) is involved in the control of cellular growth and proliferation. Abnormal activation of signaling pathways both proximal and distal to this kinase occurs frequently in human cancer suggesting that mTOR is an attractive target
Immunoregulatory functions of mTOR inhibition.
Thomson AW
Nature Reviews: Immunology, 9(5), 324-337 (2009)
mLST8 Promotes mTOR-Mediated Tumor Progression.
Kakumoto K
PLoS ONE, 10(4), e0119015-e0119015 (2015)
R Martin et al.
Cell death discovery, 2, 16024-16024 (2016-08-24)
The mammalian target of rapamycin complex 1 (mTORC1) is a highly conserved protein complex regulating key pathways in cell growth. Hyperactivation of mTORC1 is implicated in numerous cancers, thus making it a potential broad-spectrum chemotherapeutic target. Here, we characterized how
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