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SAB4200476

Sigma-Aldrich

Anti-ALIX antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-AIP1, Anti-ALG-2 interacting protein 1, Anti-DRIP4, Anti-HP95, Anti-PDCD6IP, Anti-apoptosis-linked gene 2-interacting protein X, Anti-dopamine receptor interacting protein 4, Anti-programmed cell death 6 interacting protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

mouse, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human Jurkat cells.
western blot: 2.5-5 μg/mL using whole extracts of mouse A20 cells.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PDCD6IP(10015)
mouse ... Pdcd6ip(18571)

General description

The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region.

Immunogen

peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in monkey, bovine and dog and differs by 2 amino acids in mouse and rat.

Application

Anti-ALIX antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

Biochem/physiol Actions

ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X binds to endophilins, proteins that regulate membrane shape during endocytosis. Over-expression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.
PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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ALIX is recruited temporarily into HIV-1 budding sites at the end of gag assembly.
Ku PI
PLoS ONE, 9 (2014)
Association between Programmed Cell Death 6 Interacting Protein Insertion/Deletion Polymorphism and the Risk of Breast Cancer in a Sample of Iranian Population.
Hashemi M
Disease Markers (2015)
Natália Marins Bastos et al.
PloS one, 18(1), e0280195-e0280195 (2023-01-11)
Aiming to evaluate the effects of increased body energy reserve (BER) in Nellore cows' reproductive efficiency, cows were fed with different nutritional plans to obtain animals with high BER (HBER; Ad libitum diet) and moderate BER (MBER: cows fed 70%
Paola Maria da Silva Rosa et al.
Journal of ovarian research, 17(1), 65-65 (2024-03-19)
It is well described that circulating progesterone (P4) plays a key role in several reproductive events such as oocyte maturation. However, during diestrus, when circulating P4 is at the highest concentrations, little is known about its local impact on the
Alireza Shoae-Hassani et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 40(7), 265-276 (2017-06-18)
Immune cell-derived exosomes can increase immunity against tumors. In contrast, tumor-derived exosomes can reduce the immunity and can change the tumor microenvironment to further develop and provide metastasis. These effects take place by an alteration in the innate and adaptive

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