S500HR
Sephacryl® Size Exclusion Resin
500-HR, MW range 40-20,000 kDa (dextrans)
Synonym(s):
Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)
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Product Name
Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)
Quality Level
description
stability: stable to 0.2M NaOH
form
suspension (20% aqueous ethanol)
feature
autoclavable
matrix
crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
particle size
25-75 μm (wet)
pore size
40-20,000 kDa MW range (dextrans)
compatibility
mode of use size exclusion chromatography
storage temp.
2-8°C
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General description
- Supplied swollen, suspended in 20% aqueous ethanol
- Autoclavable
- Stable to 0.2N sodium hydroxide (NaOH)
- Store at refrigerated temperature (2-8 °C)
S500HR-750ML′s updated product number is GE17-0613-01
Application
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..
Sephacryl® has been used in size exclusion chromatography purification of the Acacia tortuosa gum,[1] dextran-RBA12 from Weissella cibaria[2] and dextran from Weissella cibaria JAG8.[3]
Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
Legal Information
Sephacryl is a registered trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
100.4 - 109.4 °F - closed cup
Flash Point(C)
38 - 43 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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