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Key Documents

CL6B200

Sigma-Aldrich

Sepharose CL-6B Size Exclusion Resin

Cross-linked, 90-350 mesh

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
23151817
NACRES:
NA.84

product name

Sepharose CL-6B, Cross-linked

form

suspension

bead diameter

40-165 μm

pore size

10,000-1,000,000 fractionation range (Dextrans)
10,000-4,000,000 fractionation range (Globular proteins)

storage temp.

2-8°C

General description

Sepharose CL is a cross-linked derivative of sepharose, prepared by reacting sepharose with 2,3-dibromopropanol under strongly alkaline conditions.

Application

Sepharose CL-6B is used in affinity chromatography, protein chromatography, gel filtration chromatography, separation media and resins. Sepharose CL-6B has been used to study in vitro inhibition of human melanoma cells and to gather data in other valuable anticancer studies.
SepharoseCL-6B has been used for the purification of glucoamylase enzyme and targeted ribonucleoprotein (RNP).

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Pictograms

Flame

Signal Word

Danger

Hazard Statements

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


Certificates of Analysis (COA)

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W Chen et al.
Journal of bacteriology, 175(17), 5677-5682 (1993-09-01)
A membrane-associated ATPase with an M(r) of approximately 510,000 and containing subunits with M(r)s of 80,000 (alpha), 55,000 (beta), and 25,000 (gamma) was isolated from the methanogen Methanococcus voltae. Enzymatic activity was not affected by vanadate or azide, inhibitors of
M Schweizer et al.
Molecular & general genetics : MGG, 203(3), 479-486 (1986-06-01)
FAS1, the structural gene of the pentafunctional fatty acid synthetase subunit beta in Saccharomyces cerevisiae has been sequenced. Its reading frame represents an intron-free nucleotide sequence of 5,535 base pairs, corresponding to a protein of 1,845 amino acids with a
Ru-Ping Zhang et al.
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 30(6), 561-564 (2002-08-09)
Luffin B, a plan single-chain ribosome inactivating protein, was purified from seeds of Luffa cylindrica by Blue Sepharose CL-6B affinity chromatography. An immunotoxin was constructed with luffin B and Ng76, a monoclonal antibody to human melanoma cell M(21). Luffin B-Ng76
Amylolytic fungi in starter cakes for rice beer production
Das AJ, et al.
The Journal of General and Applied Microbiology, 63(4), 236-245 (2017)
D A Grahame
The Journal of biological chemistry, 266(33), 22227-22233 (1991-11-25)
An enzyme complex containing carbon monoxide dehydrogenase and a corrinoid protein has been isolated from Methanosarcina barkeri. Sodium dodecyl sulfate-gel electrophoresis revealed five polypeptides of molecular masses alpha = 19,700, beta = 84,500, gamma = 63,200, delta = 53,000, and

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