EXPPCRKB
Roche
KAPA Express Extract
with PCR ReadyMix
Synonym(s):
PCR, PCR extraction
About This Item
Quality Level
usage
sufficient for 100 reactions
sufficient for 500 reactions
shelf life
≤12 mo.
packaging
pkg of 1000 U (KK7152)
pkg of 200 U (KK7151)
manufacturer/tradename
Roche
technique(s)
DNA extraction: suitable
PCR: suitable
storage temp.
−20°C
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General description
The combination of KAPA Express Extract and KAPA2G Robust HotStart® ReadyMix™ provides a high performance solution for rapid DNA extraction and consistent downstream amplification.
Application
- Human tissue (formalin-fixed paraffin-embedded (FFPE) samples; blood collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples)
- Animal samples (ear or tail clippings; hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)
- Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples)
- Insects (crushed)
- Bird feathers (calamus fragments).
Features and Benefits
- Rapid extraction protocol: PCR-ready DNA in 15 minutes
- Versatility: single kit optimized for a variety of sample types
- Single-tube reaction minimizes risk of contamination
- DNA extracted with KAPA Express Extract is compatible with any PCR system
Rapid DNA extraction from a variety of sample types:
- From sample to PCR in less than 15 minutes
- Successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers
Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:
- Rapid DNA extraction and consistent downstream amplification of challenging sample types
Routine DNA extraction from blood sample types:
- Efficient extraction from “Guthrie”cards, FTA™ Elute cards, and FTA™ cards
Quick Notes:
- Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.
- Significantly reduced turnaround times and risk of sample loss and contamination.
- Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.
- One extract typically yields a sufficient amount of template for 50–500 PCRs.
- DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.
- KAPA2G Robust HotStart® ReadyMix™ is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.
- No quantification of DNA required prior to PCR.
Quality
Preparation Note
KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.
Other Notes
Legal Information
Kit Components Only
- KAPA Express Extract Enzyme 1 U/µL
- 10X KAPA Express Extract Buffer
- 2X KAPA2G Robust HotStart® ReadyMix
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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