2GFHSRMDKB
Roche
KAPA2G Fast HotStart ReadyMix
2 ×, with dye
Synonym(s):
PCR
About This Item
Quality Level
usage
sufficient for 100 reactions
sufficient for 500 reactions
shelf life
≤18 mo.
feature
Fast PCR
Multiplex PCR
dNTPs included
hotstart
packaging
kit of 1.25 mL (100 x 25 μL rxn; KK5608)
kit of 6.25 mL (500 x 25 μL rxn; KK5609)
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
PCR: suitable
input
purified DNA
storage temp.
−20°C
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General description
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.
KAPA2G Fast HotStart® DNA Polymerase is supplied in a convenient 2X ReadyMix format and is devoid of primers and template. The ReadyMix comprises KAPA2G Fast HotStart DNA Polymerase (0.5 U per 25 μL reaction) in a proprietary reaction buffer containing dNTPs (0.2 mM of each dNTP at 1X), MgCl2 (1.5 mM at 1X) along with stabilizers.
Application
- Routine PCR
- Fast PCR
- Genotyping
- Multiplex allele-specific colony-PCR (MASC-PCR)
- Genotyping polymerase chain reaction (PCR)
Biochem/physiol Actions
Features and Benefits
- Extension times as low as 1 sec per kilobase
- Broad coverage of both AT- and GC-rich targets
High speed, higher performance :
- Single copy sensitivity from complex targets
- Increased yields
Quick Notes :
- KAPA2G Fast HotStart ReadyMix PCR Kits contain the engineered KAPA2G Fast HotStart DNA Polymerase, developed for fast PCR.
- Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
- No need for specialized instrumentation or PCR consumables.
- Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
- For high reaction efficiency, do not exceed 25 μL reaction volumes.
- KAPA2G Fast HotStart ReadyMix contains 1.5 mM MgCl2 at 1X.
- KAPA2G Fast HotStart ReadyMix with dye includes two inert tracking dyes to allow direct loading onto agarose gels.
- Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.
Quality
Preparation Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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