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HomeSmall Molecule HPLCHPLC Analysis of Vitamin D2 and D3

HPLC Analysis of Vitamin D2 and D3

Elution Order: Ergocalcifero (Vitamin D2) and Cholecalciferol (Vitamin D3)

Figure 1.Elution Order: Ergocalcifero (Vitamin D2) and Cholecalciferol (Vitamin D3)

Conditions

columnAscentis® Express C18, 25 cm x 4.6 mm I.D., 5 µm particles (50538-U)
column temp.35 °C
mobile phase[A] acetonitrile; [B] methanol; (95:5, A:B)
flow rate1.5 mL/min
pressure1726 psi (119 bar)
sample50 µg/mL in 90:10, acetonitrile:methanol
injection10 µL
detectorUV, 265 nm
Table 1.Conditions

Description

Analysis NoteThis application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented.
CategoriesAnalytical Chromatography, Vitamins and Natural products, Steroids
Featured IndustryClinical
Vitamins, Nutraceuticals, and Natural Products
suitabilityapplication for HPLC
Table 2.Description
Materials
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