技術
HPLC: suitable
測試參數
column: Zenix® SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles (Z777018)
mobile phase: 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate: 0.35 mL/min
pressure: 1450 psi (100 bar)
column temp.: ambient
detector: UV, 215 nm
injection: 1 μL
sample: digested SO57 mAb, ~0.5 g/L
適合性
application for HPLC
應用
life science and biopharma
分析報告
Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc, after digestion with IdeS protease
法律資訊
Zenix is a registered trademark of Sepax Technologies
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