ZRB939

Anti-Pancreatic Polypeptide Antibody, clone 3H5, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Obinepitide, PP, Pancreatic Prohormone
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3H5, recombinant monoclonal; monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 10.44 kDa
• 物種活性: human
• 物種活性（以同源性預測）: bovine
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable using 1:100; immunofluorescence: 1:100-1:400; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400; western blot: suitable using 1:1,000
• 同型: IgG
• UniProt登錄號: P01298
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PPY;PNP(5539)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 3H5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Pancreatic Polypeptide.

免疫原

KLH-conjugated linear peptide corresponding to full-length human Pancreatic Polypeptide (without the signal and propeptides).

應用

Anti-Pancreatic Polypeptide, clone 3H5 ZooMAb, Cat. No. ZRB939, is a recombinant Rabbit Monoclonal Antibody that detects pancreatic polypeptide and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.

Immunofluorescence Analysis: A 1:400 dilution from a representative lot detected Pancreatic Polypeptide in human pancreas tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Pancreatic Polypeptide in human pancreas tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Pancreatic Polypeptide in TGP52 cell line.

標靶描述

Pancreatic polypeptide (UniProt: P01298; also known as PP, Obinepitide, Pancreatic prohormone) is encoded by the PPY (also known as PNP) gene (Gene ID: 5539) in human. Pancreatic polypeptide is a member of the neuropeptide Y family and is secreted protein that is produced by PP cells in the pancreatic islets of Langerhans. The pancreatic prohormone is synthesized with a signal peptide (aa 1-29) and a propeptide (aa 89-95) that are subsequently cleaved off. The remaining part is cleaved off into pancreatic hormone (aa 30-65) and pancreatic icosapeptide (69-88). Pancreatic hormone acts as a regulator of pancreatic and gastrointestinal functions. Two isoforms of pancreatic polypeptide have been described that are generated by alternative splicing. Pancreatic polypeptide release is functionally associated with increased satiety, delayed gastric emptying, inhibition of gallbladder motility, and regulation of energy expenditure. Its deficiency is observed in cases of pancreatic resection and chronic pancreatitis and is associated with significant reduction in PP cell mass and abnormally high hepatic glucose production even in the presence of endogenous insulin. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Maxwell, J et al. (2014). Pancreas 43(4); 651-656).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable