ZRB2396

Anti-MST1/STK4 Antibody, clone 1L17-L2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: EC: 2.7.11.1, Mammalian STE20-like protein kinase 1, STE20-like kinase MST1, Serine/threonine-protein kinase 4, Serine/threonine-protein kinase Krs-2
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1L17-L2, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: mouse, rat
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal
• Protein ID登錄號: NP_006273
• UniProt登錄號: Q13043
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... STK4(6789)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1L17-L2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Serine/threonine-protein kinase 4 (MST1/STK4). It targets an epitope within 16 amino acids from the N-terminal region. Detects the intact and cleaved forms.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Serine/threonine-protein kinase 4 (MST1/STK4).

應用

Quality Control Testing

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MST1/STK4 in Ramos cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MST1/STK4 in Caco2 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound MST1/STK4 peptide with a KD of 1.8x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MST1/STK4 in Human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MST1/STK4 in Ramos cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Serine/threonine-protein kinase 4 (UniProt: Q13043; also known as EC: 2.7.11.1, Mammalian STE20-like protein kinase 1, MST-1, STE20-like kinase MST1, Serine/threonine-protein kinase Krs-2, STK4) is encoded by the STK4 (also known as KRS2, MST1) gene (Gene ID: 6789) in human. STK4 is a homodimeric, stress-activated, pro-apoptotic kinase that enters the nucleus following its caspase cleavage and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is ubiquitously expressed with higher expression observed in prostate cancer and its levels increase with the progression to malignant state. Its protein kinase domain is localized in residues 30-281. It is activated by caspase-cleavage between amino acids 326-327 and 349-350. The caspase-cleaved form cycles between the nucleus and cytoplasm. Its full activation requires homodimerization and autophosphorylation at threonine 183. Its activity is inhibited by the C-terminal non-catalytic region. STK4 is a KEY^® component of the Hippo signaling pathway, which plays a pivotal role in organ size control and acts as a tumor suppressor by restricting cell proliferation and promoting apoptosis. During apoptosis, it phosphorylates serine 14 of histone H2B and phosphorylates FOXO3 upon oxidative stress that results in its NuCLEAR^™ translocation and initiation of cell death. Its phosphorylation at threonine 387 by AKT1, LEADS^™ to inhibition of its kinase activity, NuCLEAR^™ translocation and autophosphorylation at threonine 183. It also reduces its cleavage by caspases and its ability to phosphorylate FOXO3. Two isoforms of STK4 have been described that are produced by alternative splicing. Mutations in STK4 gene have been linked to T cell immunodeficiency resulting from progressive loss of naive T-cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Guennoun, A., et al. (2021). J. Clin. Immunol. 41(8); 1839-1852; Praskova, M., et al. (2004). Biochem. J. 381(Pt2); 453-462; Lee, KK., et al. (2001). J. Biol Chem. 276(22); 19276-19285).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

Affinity is a trademark of Mine Safety Appliances Co.

KEY is a registered trademark of Hanna Instruments

LEADS is a trademark of Compugen

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable