ZRB1995

Anti-MAD2L1 Antibody, clone 1E9 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Mitotic spindle assembly checkpoint protein MAD2A;HsMAD2;Mitotic arrest deficient 2-like protein 1;MAD2-like protein 1
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1E9, recombinant monoclonal
• 描述: 1E9 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 23.51 kDa; observed mol wt ~24 kDa
• 純化經由: using Protein A
• 物種活性: human, rat
• 物種活性（以同源性預測）: porcine, monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_002349
• UniProt登錄號: Q13257
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MAD2L1(4085)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1E9 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Mitotic spindle assembly checkpoint protein MAD2A (MAD2). It targets an epitope within 18 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human Mitotic spindle assembly checkpoint protein MAD2A.

應用

Quality Control Testing

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MAD2L1 in L6 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MAD2L1 in HeLa and A431 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MAD2L1 in human ovarian cancer tissue sections.

Affinity Binding Assay Analysis: A representative lot of this antibody bound Mad2L1 peptide with a KD of 1.8 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Mitotic spindle assembly checkpoint protein MAD2A (UniProt: Q13257; also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2) is encoded by the MAD2L1 (also known as MAD2) gene (Gene ID: 4085) in human. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It exists as a monomer or homodimer. It is reported to exist either in an open and inactive (O-MAD2) conformation or in the closed, active (C-MAD2) conformation. In the closed conformation it forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2. MAD2L1 undergoes phosphorylation on multiple serine residues by NIMA related kinase 2 (NEK2). The level of phosphorylation varies during the cell cycle and is shown to be the highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Two isoforms of MAD2L1 are described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ji, W., et al. (2018). J. Biol. Chem. 293(2); 484-496; Mapelli, M., et al. (2007). Cell. 131(4); 730-743; Luo, X., et al. (2000). Nat. Struct. Biol. 7(3); 224-229).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable