ZRB1920

Anti-Nanog Antibody, clone 4D17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Homeobox protein NANOG;ES cell-associated protein 4;Early embryo specific expression NK-type homeobox protein;Homeobox transcription factor Nanog
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4D17, recombinant monoclonal
• 描述: 4D17 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 31.86 kDa; observed mol wt ~37 and 32 kDa
• 純化經由: using Protein A
• 物種活性: mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal half
• Protein ID登錄號: NP_082292
• UniProt登錄號: Q80Z64
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Nanog(71950)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 4D17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Nanog. It targets an epitope within 20 amino acids from the N-terminal half. Predicted to react with both isoforms.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of mouse Nanog.

應用

Quality Control Testing

Evaluated by Western Blotting in E14-TG2a cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nanog in E14-TG2a cell lysates.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nanog in Mouse embryonic stem cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nanog in mouse testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nanog in E14-TG2a cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Homeobox protein NANOG (UniProt: Q80Z64; also known as ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog) is encoded by the Nanog (also known as Ecat4, Enk) gene (Gene ID: 71950) in murine species. Nanog is a transcription factor that is involved in the self-renewal of embryonic stem cells (ES) and is a critical factor for the maintenance of the undifferentiated state of pluripotent cells. It binds optimally to the DNA consensus sequence 5′-TAAT[GT][GT]-3′ or 5′-[CG][GA][CG]C[GC]ATTAN[GC]-3′. Its expression is observed in inner cell mass (ICM) of the blastocyst and gonocytes and its expression decreases with ES differentiation. Nanog can autorepress its own expression in differentiating ES cells by binding to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. Overexpression of Nanog in ES cells maintains specific differentiation, however, its overexpression in cancer stem cells is reported to inhibit apoptosis. Together with OCT4, it can activate SNAIL2 and promote metastasis and the EMT phenotype in cancer cells. Nanog undergoes phosphorylation on serine 130 and 131 by casein kinase I and phosphomimetic substitution of serine 130 is shown to abolish its DNA binding and eliminates LIF‐independent self‐renewal. On the other hand, phosphomimetic substitution of serine 131 enhances LIF‐independent self‐renewal but does not affect its DNA binding. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mullin, NP., et al. (2021). FEBS Lett. 595(1); 14-25; Gawlik-Rzemieniewska, N., and Bednarek, I (2016). Cancer Biol. Ther. 17(1); 1-10; Kim, S-H., et al. (2014). Stem Cell Res. 13(1); 1-11).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable