ZRB1606

Anti-Raf-1 Antibody, clone 1I3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: RAF proto-oncogene serine/threonine-protein kinase;EC:2.7.11.1;Proto-oncogene c-RAF;cRaf
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1I3, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 73.05 kDa; observed mol wt ~85 kDa
• 純化經由: using Protein A
• 物種活性: human, mouse, rat
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal
• Protein ID登錄號: NP_002871
• UniProt登錄號: P04049
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RAF1(5894)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1I3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Raf-1. It targets an epitope within 19 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human Raf-1.

應用

Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Raf-1 in U2OS cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Raf-1 in C2C12, PC12, and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Raf-1 in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Raf-1 peptide with a KD of 2.5 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

RAF proto-oncogene serine/threonine-protein kinase (UniProt: P04049; also known as EC:2.7.11.1, Proto-oncogene c-RAF, cRaf, Raf-1) is encoded by the RAF1 (also known as RAF) gene (Gene ID: 5894) in human. Raf-1 is a serine/threonine protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAP kinase cascade. Its regulation is a highly complex process that involves membrane recruitment, protein-protein interactions, dimerization, and phosphorylation/dephosphorylation events. It functions as a critical regulatory switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival, and oncogenic transformation. Raf-1 activation initiates a MAP kinase cascade that includes sequential phosphorylation of MEK1/2 and ERK1/2. In its inactive conformation, Raf-1 is maintained by autoinhibitory interactions occurring between the N-terminal regulatory and the C-terminal catalytic domains and by the binding of a 14-3-3 protein that contacts two phosphorylation sites, serine 259 and 621. Upon mitogenic stimulation, Ras and PPP2R1A cooperate to release autoinhibition and the subsequent phosphorylation of activating sites that include serine 338, tyrosine 341, threonine 491, and serine 494 result in its full activation. Through a negative feedback mechanism involving ERK2, Raf-1 is phosphorylated on multiple serine residues (Serine 29, 43, 289, 296, 301, and 642) to yield an inactive, desensitized kinase. Two isoforms of Raf-1 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, J., et al. (2001). Proc. Natl. Acad. Sci. USA. 98(14); 7783-7788; Chong, H., et al. (2001). EMBO J. 20(14); 3716-3727).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable