ZRB1521

Anti-MDR-1 Antibody, clone 1O12 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: ATP-dependent translocase ABCB1, CD243, Multidrug resistance protein 1, P-glycoprotein 1, Phospholipid transporter ABCB1, also known as ATP-binding cassette sub-family B member 1
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1O12, monoclonal; recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 141.48 kDa; observed mol wt ~150 kDa
• 物種活性: human, mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: P08183
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ABCB1(5243)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1O12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects MDR1 protein. It targets an epitope within 22 amino acids from internal, cytoplasmic loop region.

免疫原

KLH-conjugated linear peptide corresponding to 22 amino acids from the internal cytoplasmic loop region of human Multidrug resistance protein 1 (MDR-1).

應用

Anti-MDR-1, clone 1O12 ZooMAb, Cat. No. ZRB1521, is a recombinant Rabbit monoclonal antibody that targets MDR-1 and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDR-1 in HepG2 and NIH 3T3 cells.

Affinity Binding Assay: A representative lot bound MDR-1 peptide with a KD of 3.5 x 10-7 in an affinity binding assay.

Flow Cytometry Analysis: 0.01 μg from a representative lot detected MDR-1 in HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MDR-1 in human adrenal gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Multidrug resistance protein 1 (UniProt: P08183; also known as ATP-binding cassette sub-family B member 1, ATP-dependent translocase ABCB1, P-glycoprotein 1, Phospholipid transporter ABCB1, CD243; MDR1) is encoded by the ABCB1 (also known as MDR1, PGY1) gene (Gene ID: 5243) in human. In tumor cells, the classical MDR phenotype is conferred by MDR1 that is expressed in about 50% of human cancers. MDR1 is a multi-pass membrane N-linked glycoprotein that translocates drugs and phospholipids across the membrane. It contains 6 extracellular domains, 12 transmembrane domains, and 7 cytoplasmic domains. Its ABC transporter domains are localized to amino acids 392-628 and 1035-1273.MDR1 expression has been reported in liver, kidney, small intestine, and brain. It catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. It participates mainly to the flop of phosphatidylcholine, phosphatidyl-ethanolamine, beta-D-glucosylceramides and sphingomyelins. It serves as an energy-dependent efflux pump that is responsible for decreased drug accumulation in multidrug-resistant cells. Overexpression of MDR1 is shown to be of prognostic value in leukemia, neuroblastoma and sarcoma and its inhibition can restore chemosensitivity in tumor cells. Mutations in ABCB1 gene has been linked to inflammatory bowel disease 13, a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Perez, J., et al. (2011). Anticancer Res. 31(9); 2813-2820; Kaszubiak, A., et al. (2007). Int. J. Oncology 31(2); 419-430).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1