推薦產品
生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
2E17, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 37.56 kDa
observed mol wt ~50 kDa
物種活性
human, mouse
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... CEBPA(1050)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 2E17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects C-EBP alpha. It targets an epitope within 147 amino acids from the N-terminal region.
免疫原
GST/His-tagged recombinant fragment corresponding to 147 amino acids from N-terminal region of human C-EBP alpha, isoform 1.
應用
Anti-C-EBP alpha, clone 2E17 ZooMAb, Cat. No. ZRB1367, a recombinant Rabbit monoclonal antibody that specifically targets C-EBP alpha and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected C-EBP alpha in THP-1 cell lysate.
Flow Cytometry Analysis: 0.01 μg from a representative lot detected C-EBP alpha in one million U937 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected C-EBP alpha in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Flow Cytometry Analysis: 0.01 μg from a representative lot detected C-EBP alpha in one million U937 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected C-EBP alpha in THP-1 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
CCAAT/enhancer-binding protein alpha (UniProt: P49715; also known as C/EBP alpha, C-EBP alpha) is encoded by the CEBPA (also known as CEBP) gene in human. C-EBP alpha belong to the bZIP family of proteins that serves as a transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. It binds directly to the consensus DNA sequence 5′-T[TG]NNGNAA[TG]-3′ acting as an activator on distinct target genes. It can bind DNA as a homodimer or as a stable heterodimer with CEBPB, CEBPD, CEBPE, and CEBPG. It is reported to be essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP) and has also been shown to be critical for the proper development of the liver and the lung and for terminal adipocyte differentiation. C-EBP alpha down-regulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. It can be phosphorylated on multiple sites. Phosphorylation at serine 190 is shown to be essential for its interaction with CDK2, CDK4 and SWI/SNF complex leading to cell cycle inhibition. Phosphorylation at threonine 226 and threonine 230 by GSK3 is constitutive in adipose and lung tissue and these sites are also shown to be phosphorylated in liver during feeding, but not during fasting cycle. Four isoforms of C-EBP alpha have been described that are produced by alternative splicing. Mutations in CEBPA gene are known to cause acute myelogenous leukemia (AML). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wedel, A., et al. (1995). Immunobiology. 193(2-4); 171-185; Johnson, PF et al. (2005). J. Cell Sci. 118(12); 2545-2555).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
分析證明 (COA)
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