推薦產品
生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1F23, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 24.0 kDa
observed mol wt ~26 kDa
物種活性
human, rat, mouse
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... TNNI1(7135)
相關類別
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1F23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects cardiac troponin I. It targets an epitope within 14 amino acids from the C-terminal region.
免疫原
KLH-conjugated liner peptide corresponding to 14 amino acids from the C-terminal region of human Cardiac Troponin I.
應用
Anti-Cardiac Troponin I, clone 1F23 ZooMAb, Cat. No. ZRB1355, a Rabbit monoclonal antibody that targets Cardiac Troponin I and is tested in Immunocytochemisty, Immunofluorescence, Immunohistochemistry and Western Blotting.
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Cardiac Troponin I in human heart tissue lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cardiac Troponin I in AC16 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin I in human heart and rat heart tissue sections.
Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected Cardiac Troponin I in human heart muscle tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cardiac Troponin I in AC16 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin I in human heart and rat heart tissue sections.
Immunofluorescence Analysis: A 1:1000 dilution from a representative lot detected Cardiac Troponin I in human heart muscle tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Troponin I, cardiac muscle (UniProt: P19429; also known as Cardiac troponin I) is encoded by the TNNI3 (also known as TNNC1) gene (Gene ID: 7137) in human. Troponin is a complex that regulates the contraction of skeletal muscle. It consists of 3 subunits known as C, T, and I, which are located periodically along the thin filament of the myofibrils. Troponins are found in skeletal and cardiac muscle, but not in smooth muscle. Troponin C (TnC) binds calcium ions, Troponin I is the inhibitory subunit of troponin that inhibits actomyosin ATPase and Troponin T binds to tropomyosin. It also confers calcium-sensitivity to actomyosin ATPase. Amino acids region 32-79 of Troponin I is involved in binding to TnC and region 129-149 is involved in binding to both TnC and actin. Cardiac muscle Troponin I has 31 amino acid residues at its N-terminal that are not present in the skeletal muscle form. Hence, cardiac specificity of this isoform is shown to offer accuracy in diagnosis in acute or chronic skeletal muscle injury. Mutation in TNNI3 gene are reported to result in multiple types of cardiomyopathies that can result in premature death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, H-C., and Fajer, PG (1998). Biochemistry 37(19); 6628-6635; Skeik, N., and Patel, DC (2007). Int. J. Angiol. 16(2); 53-58).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Jara M Baena-Montes et al.
Journal of cardiovascular development and disease, 9(8) (2022-08-26)
Cardiac ablation with irreversible electroporation (IRE) is quickly being established as a modality of choice for atrial fibrillation treatment. While it has not yet been optimised, IRE has the potential to significantly limit collateral damage and improve cell-specific targeting associated
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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