ZRB1353

Anti-ZNF598 Antibody, clone 3N7, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: E3 ubiquitin-protein ligase ZNF598, EC 2.3.2.27, Zinc finger protein 598
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3N7, monoclonal; recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 98.64 kDa; observed mol wt ~110 kDa
• 物種活性: human, mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: Q86UK7
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ZNF598(90850)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 3N7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects E3 ubiquitin-protein ligase ZNF598. It targets an epitope from the internal region.

免疫原

His-tagged recombinant fragment corresponding to 250 amino acids from the internal region of human E3 ubiquitin-protein ligase ZNF598, isoform 1.

應用

Anti-ZNF598, clone 3N7 ZooMAb, Cat. No. ZRB1353, recombinant Rabbit monoclonal antibody that targets E3 ubiquitin-protein ligase ZNF598 and has been tested in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ZNF598 in human pancreas tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZNF598 in A431, HeLa, HEK293, and NIH 3T3 cells.

Western Blot Analysis: A representative lot of this antibody detected ZNF598 in U2OS wild type and U2OS CRISPR KO cells (1:2000 dilution) and in mouse embryonic fibroblasts (1:500 dilution).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

E3 ubiquitin-protein ligase ZNF598 (UniProt: Q86UK7; also known as EC:2.3.2.27, Zinc finger protein 598) is encoded by the ZNF598 gene (Gene ID: 90850) in human. ZNF598 is a E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation. It is required for ribosomes to terminally stall during translation of poly(A) sequences by mediating monoubiquitination of 40S ribosomal protein RPS10/eS10, RPS20/uS10 and RPS3/uS3. It also acts as a component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation. ZNF598 has one RING type (aa 29-69) and a C2H2 type (aa 187-210) zinc finger domain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Garzia, A., et al. (2017). Nat. Commun. 8, 16056).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable