ZRB1318

Anti-Pericentrin Antibody, clone 2H11, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Kendrin, Pericentrin-B
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2H11, monoclonal; recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 378.04 kDa; observed mol wt ~380 kDa
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: O95613
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PCNT(5116)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 2H11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Pericentrin. It targets an epitope within 132 amino acids from the N-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 132 amino acids from the N-terminal region of human Pericentrin.

應用

Anti-Pericentrin, clone 2H11 ZooMAb, Cat. No. ZRB1318, a Rabbit monoclonal antibody that targets human Pericentrin and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Pericentrin in HeLa, A431, HUVEC and HepG2 cell lines.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Pericentrin with a KD of 2.7 x 10-9 in an affinity binding assay.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Pericentrin in HeLa and HEK293 cell lysates.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Pericentrin (UniProt: O95613; also known as Kendrin, Pericentrin-B) is encoded by the PCNT (also known as KIAA0402, PCNT2) gene (Gene ID: 5116) in human. Pericentrin is a highly conserved protein of the pericentriolar material and is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. The centrosomal localization of pericentrin is shown to be mediated by a pericentrosomal matrix targeting motif, known as PACT domain, in its C-terminal region. Pericentrin plays a role, together with DISC1, in the microtubule network formation. It is also reported to prevent premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. It is also involved in retinal development by regulating photoreceptor ciliogenesis and photoreceptive outer segment formation and plays a role in ciliary development in olfactory receptor neurons. Pericentrin is shown to bind to calmodulin by a C-terminal binding site (aa 3195-3208) and is essential for its functions at centrosomes. During mitosis, pericentrin is cleaved to remove CDK5RAP2 that promotes centriole disengagement and subsequent centriole separation. Mutations in PCNT2 gene are known to cause microcephalic osteodysplastic primordial dwarfism that is characterized by microcephaly and short stature. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Falk, N., et al. (2018). J. Cell Sci. 131(16); jcs218487).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable