生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1H21, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 23.72 kDa
observed mol wt ~34 kDa
物種活性
human
物種活性（以同源性預測）
monkey
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
ELISA: suitable
immunocytochemistry: suitable
同型
IgG
表位序列
Extracellular domain
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相關類別
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1H21 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human T-cell surface glycoprotein CD8 beta chain (CD8β). It targets an epitope within 13 amino acids from the extracellular domain.
免疫原
KLH-conjugated linear peptide corresponding to 13 amino acids from the extracellular domain of human T-cell surface glycoprotein CD8 beta chain (CD8β).
應用
Quality Control Testing
Evaluated by Western Blotting in PBMC cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD8β in peripheral blood mononuclear cell (PBMC) lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD8β in human thymus and human spleen tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD8β in human thymus tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CD8β peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Flow Cytometry Analysis: 1 βg from a representative lot detected CD8β in one million Hut78 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD8β in Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in PBMC cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD8β in peripheral blood mononuclear cell (PBMC) lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD8β in human thymus and human spleen tissue lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD8β in human thymus tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CD8β peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Flow Cytometry Analysis: 1 βg from a representative lot detected CD8β in one million Hut78 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD8β in Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CD8β, clone 1H21 ZooMAb®, Cat. No. ZRB1255, is a recombinant Rabbit monoclonal antibody that targets CD8β and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
標靶描述
T-cell surface glycoprotein CD8 beta chain (UniProt: P10966; also known as CD8b) Is encoded by the CD8B (also known as CD8B1) gene (Gene ID: 926) in human. CD8β is a single-pass type I membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-170), a transmembrane domain (aa 171-191), and a cytoplasmic domain (aa 192-210). Eight different isoforms of CD8β have been described that are generated by alternative splicing. Isoforms 1,3,5,6,7,8 are expressed in both thymus and peripheral CD8+ T cells. For efficient cell surface expression, it requires the CD8a as a partner and the heterodimer CD8a/CD8β localizes to lipid rafts due to CD8β cytoplasmic tail palmitoylation. In T-cells, it functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. It interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
分析證明 (COA)
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