ZRB1204

Anti-p300 Antibody, clone 4E5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4E5, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 264.16 kDa; observed mol wt ~280 kDa
• 純化經由: using Protein A
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal half
• Protein ID登錄號: NP_001420.2
• UniProt登錄號: Q09472
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 4E5 a ZooMAb^® Rabbit recombinant monoclonal antibody that detects Histone acetyltransferase p300. It targets an epitope within the N-terminal half.

免疫原

His-tagged recombinant fragment corresponding to 233 amino acids from the N-terminal half of human Histone acetyltransferase p300.

應用

Quality Control Testing

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected p300 in MCF-7 cells.

Tested applications

ELISA Analysis: 300 μg/mL from a representative lot detected p300 in an ELISA.

Affinity Binding Assay: A representative lot of this antibody bound p300 with a KD of 2 x 10-7 in an affinity binding assay.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected p300 in nuclear extracts from HeLa and NTERA2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-p300, clone 4E5 ZooMAb^®, Cat. No. ZRB1204, is a recombinant Rabbit monoclonal antibody that detects p300 (Histone Acetyltransferase p300) and is tested for use Affinity Binding Assay, ELISA, Immunocytochemistry, and Western Blotting.

標靶描述

Histone acetyltransferase p300 (UniProt: Q09472; also known as EC:2.3.1.48, p300 HAT, E1A-associated protein p300, Histone butyryltransferase p300, Histone crotonyltransferase p300, Protein 2-hydroxyisobutyryltransferase p300, Protein propionyltransferase p300) is encoded by the EP300 (also known as P300) gene (Gene ID: 2033) in human. p300 serves as a transcriptional co-activator protein that plays a central role in coordinating and integrating multiple signal-dependent transcriptional events, such as cell proliferation, differentiation, and apoptosis. It has histone acetyltransferase (HAT) activity that transfers an acetyl group to the -amino group of multiple lysine residues. p300 localizes to active chromatin and acetylates all four core histones in nucleosomes, providing an epigenetic tag for transcriptional activation. It also shown to mediate cAMP-gene regulation by binding specifically to phosphorylated CREB protein. It mediates acetylation of histone H3 at lysine 122, a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. It also mediates acetylation of histone H3 at lysine 27. It can also acetylase a number of non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2. Its acetylation of HDAC1 is shown to lead to inactivation and modulation of transcription. p300 contains one TAZ-type 1 (aa 331-417), one TAZ-type 2 (aa 1728-1809), and one ZZ-type (aa 1664-1707) zinc finger domains. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable