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Sigma-Aldrich

SYBR® Green Extract-N-Amp™组织PCR试剂盒

sufficient for 1000 extractions, sufficient for 1000 amplifications

同義詞:

Tissue direct PCR kit, Tissue direct real-time PCR

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About This Item

分類程式碼代碼:
41106303
NACRES:
NA.55

用途

sufficient for 1000 amplifications
 sufficient for 1000 extractions
 sufficient for 1000 reactions

特點

dNTPs included
hotstart

技術

PCR: suitable

儲存溫度

−20°C

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相關類別

一般說明

The SYBR® Green Extract-N-Amp™ Tissue PCR kit is a direct PCR (polymerase chain reaction) kit that contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

應用

SYBR® Green Extract-N-Amp™ tissue PCR kit has been used for DNA extraction and genotyping.

特點和優勢

  • Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR)
  • Fast – tissue to qPCR in 15 minutes
  • Convenient – no long enzymatic digestions and no column purifications
  • Simple – rapid, easy-to-follow protocol
  • Sensitive – specially formulated Hot Start® SYBR® Green PCR ReadyMix™ for highly specific PCR amplification and quantitation of genomic DNA
  • Safe – no organic extraction with hazardous chemicals

成分

试剂盒含有Extraction Solution、Neutralization Solution、Tissue Preparation Solution和Extract-N-Amp SYBR Green PCR ReadyMix™。Extract-N-Amp SYBR Green PCR ReadyMix为2X反应混合物，其中含有 SYBR Green、缓冲液、盐、dNTP、Taq聚合酶和 JumpStart™ Taq 抗体。此试剂盒专为结合提取试剂使用进行了优化，其中含有适用于热启动PCR、可增强特异性的 JumpStart Taq抗体，以及可用作实时定量PCR的非特异性报告标志物的SYBR Green I。

原則

DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances and the extract is ready for real-time PCR in any plate-based real-time thermal cycling system. An aliquot of the neutralized extract is then combined with the Extract-N-Amp™ SYBR® Green PCR ReadyMix™ and user-provided PCR primers.

其他說明

更多信息，请见www.sigma-aldrich.com/extract-n-amp。

法律資訊

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056和6,171,785。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的，仅此等数量的产品用于购买者内部研究用途的的免责许可。其他专利声明下的任何权利（如美国专利号6,814,934号下的设备和系统专利声明）、进行任何专利方法申请的权利、进行任何形式的商业服务的权利，包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。

依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

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產品號碼
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訊號詞

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危險分類

Aquatic Chronic 2 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

10 - Combustible liquids

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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文章

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

基因組 DNA 的純化與評估

簡單的 DNA/RNA 純化方法有助於從各種來源進行基因組分析，提高研究效率。

條款

Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit

The REDExtract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

SYBR® Green Extract-N-Amp™ PCR Kit

SYBR® Green Extract-N-Amp™ Tissue PCR Kit enables rapid genomic DNA extraction and amplification from animal tissues.

Extract-N-Amp™ Tissue PCR Kit Protocol

The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

相關內容

Genomic DNA Extraction and Amplification Procedure using REDExtract-N-Amp™ Tissue PCR Kit

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SYBR® Green Extract-N-Amp™ Tissue PCR Kit Protocol

The SYBR® Green Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

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