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Merck
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U7883

Sigma-Aldrich

Monoclonal Anti-USP25 antibody produced in mouse

~1.0 mg/mL, clone U25.7, purified immunoglobulin, buffered aqueous solution

同義詞:

Anti-USP21, Anti-Ubiquitin specific peptidase 25

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

U25.7, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~126 kDa

物種活性

rat, mouse, human

濃度

~1.0 mg/mL

技術

immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using total extract of HEK-293T cells expressing USP25.

同型

IgG2a

UniProt登錄號

Q9UHP3

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... USP25(29761)
mouse ... Usp25(30940)
rat ... Usp25(304150)

一般說明

Monoclonal Anti-USP25 (mouse IgG2a isotype) is derived from the hybridoma U25.7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human USP25. USP25 (ubiquitin-specific protease 25) is a member of the ubiquitin processing proteases (UBP) family. It is located on the gene-poor region of 21q11.2 human chromosome.

應用

Monoclonal Anti-USP25 antibody has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry

生化／生理作用

USP25 (ubiquitin-specific protease 25) is a target for sumoylation. It takes place within the two ubiquitin interaction motifs (UIM) of USP25 that are required for efficient hydrolysis of Ub (ubiquitin), and thus impairs USP25 activity as a result of reduced affinity to Ub chains. Overexpression of USP25 has been observed in Down syndrome fetal brain.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析證明 (COA)

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Erik Meulmeester et al.
Molecular cell, 30(5), 610-619 (2008-06-10)
Vertebrates express two distinct families of SUMO proteins (SUMO1 and SUMO2/3) that serve distinct functions as posttranslational modifiers. Many proteins are modified specifically with SUMO1 or SUMO2/3, but the mechanisms for paralog selectivity are poorly understood. In a screen for
A Bosch-Comas et al.
Cellular and molecular life sciences : CMLS, 63(6), 723-734 (2006-02-28)
The biological functions of the more than one hundred genes coding for deubiquitinating enzymes in the human genome remain mostly unknown. The USP25 gene, located at 21q11.2, encodes three protein isoforms produced by alternative splicing. While two of the isoforms

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