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T8819

Sigma-Aldrich

TFIIB human

~5 units/μL, recombinant, expressed in E. coli, solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.56

重組細胞

expressed in E. coli

品質等級

形狀

solution

分子量

~32 kDa

濃度

~5 units/μL

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... GTF2B(2959)

生化/生理作用

General transcription factor for RNA Polymerase II.
The amino terminal region of TFIIB contains a zinc ribbon fold composed of antiparallel Β strands. Mutational studies show that this domain is required for recruitment of RNA Pol II for transcriptional initiation.

單位定義

One gel shift unit is the amount of TFIIB required to form 0.2 pmol of TFIIB/TFIID/DNA complex as detected by gel shift.

外觀

Solution in 20 mM Tris-HCl, pH 7.4, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 0.1% Triton® X-100.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


分析證明 (COA)

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S Hahn et al.
Genes & development, 14(6), 719-730 (2000-03-25)
The function of the conserved zinc-binding domains in the related Pol II- and Pol III-specific factors TFIIB and Brf was investigated. Three-dimensional structure modeling and mutagenesis studies indicated that for both factors, the functional surface of the zinc ribbon fold

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