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重要文件

T7791

Sigma-Aldrich

胸腺嘧啶核苷5′-三磷酸 钠盐 溶液

10 mM (pH 7.0)

同義詞:

TTP

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
41106305
PubChem物質ID:
NACRES:
NA.52

化驗

≥99%

品質等級

形狀

liquid

濃度

10 mM (pH 7.0)

顏色

colorless

異物活動

DNase, RNase, none detected

運輸包裝

dry ice

儲存溫度

−20°C

SMILES 字串

[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

InChI 密鑰

KAELIXTVSANDSE-UHFFFAOYSA-N

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相關類別

一般說明

胸苷5′-三磷酸钠盐溶液可用于DNA合成反应,如 PCR、DNA 测序、cDNA合成和分子克隆技术。

應用

Thymidine 5′-triphosphate sodium salt solution has been used:

  • as a standard to quantify each dNTP from cellular lysates by the liquid chromatography-mass spectrometry (LC-MS/MS) method
  • as a component of the dNTP mix for labeling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe
  • for routine PCR amplifications

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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Xinhui Chen et al.
Biomedical chromatography : BMC, 31(3) (2016-08-25)
The endogenous deoxynucleoside triphosphate (dNTP) pool includes deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP) and thymidine triphosphate (TTP). The endogenous dNTP pool is regulated by complex enzymatic pathways that can be targeted by drugs, such as antimetabolites. In
Michael G Mohsen et al.
Nucleic acids research, 47(18), 9495-9501 (2019-09-11)
We document the preparation and properties of dimerized pentaphosphate-bridged deoxynucleotides (dicaptides) that contain reactive components of two different nucleotides simultaneously. Importantly, dicaptides are found to be considerably more stable to hydrolysis than standard dNTPs. Steady-state kinetics studies show that the

條款

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

相關內容

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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