SRP6439

CD62E/E-Selectin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 同義詞: E-Selectin, ELAM1, ESEL, LECAM2, RP1-117P20.2, SELE
• 分類程式碼代碼: 12352200

屬性

• 生物源: human
• 重組細胞: expressed in HEK 293 cells
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: calculated mol wt 60 kDa; observed mol wt 110 kDa by SDS-PAGE (DTT-reduced. Trp22 is the predicted N-terminal.)
• 包裝: pkg of 100 μg; pkg of 20 μg
• UniProt登錄號: P16581
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... SELE(6401)

描述

一般說明

Selectins are a family of calcium-dependent type 1 transmembrane proteins. Endothelial (E)-selectin is a heavily glycosylated transmembrane protein expressed by activated endothelial cells in microvascular linings. E-selectin consists of an N-terminal type 1 lectin domain, an EGF-like domain, 6 sushi (CCP/SCR) domains, a transmembrane sequence, and a short cytoplasmic domain. Recombinant human E-selectin is a 58.6kDa protein containing 535 amino acid residues, corresponding to the extracellular portion of the full length protein. Due to glycosylation, E-selectin migrates at an apparent molecular weight of approximately 65-85kDa by SDS-PAGE analysis under reducing conditions. The gene encoding this protein is localized on human chromosome 1q24.2.

生化／生理作用

E-selectin, along with P-selectin and L-selectin, initiate recruitment of circulating leukocytes from blood to sites of inflammation in the vascular lining through interaction with specific cell surface associated carbohydrate determinants. It is a pro-angiogenic factor. This protein has also been implicated in the pathogenesis of stroke and atherosclerosis.

外觀

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

重構

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable