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Merck
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SRP3019

Sigma-Aldrich

BRAK (CXCL14) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

同義詞:

Breast and Kidney-expressed chemokine, CXCL14, NJAC, bolekine

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in E. coli

化驗

≥98% (HPLC)
≥98% (SDS-PAGE)

形狀

lyophilized

效力

1.0-10.0 ng/mL

分子量

9.4 kDa

包裝

pkg of 20 μg

技術

cell culture | mammalian: suitable

雜質

<0.1 EU/μg endotoxin, tested

顏色

white to off-white

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... CXCL14(9547)

一般說明

Breast and Kidney-expressed chemokine (BRAK) is a CXC chemokine expressed in normal tissue in the absence of inflammatory stimuli, and infrequently expressed in cancer cell lines. BRAK is known to be a highly selective monocyte chemoattractant. However, main function and receptor selectivity is unknown at this time. BRAK contains the four highly conserved cysteine residues present in CXC chemokines. The sequence of the mature protein consists of 87 amino acid residues, and is approximately 30% homologous to the sequences of MIP-2 alpha and beta. Recombinant human BRAK is a 9.4 kDa protein containing 77 amino acid residues.

生化/生理作用

Breast and Kidney-expressed chemokine (BRAK) is a CXC chemokine expressed in normal tissue in the absence of inflammatory stimuli, and infrequently expressed in cancer cell lines. Recombinant human BRAK is a 9.4 kDa protein containing 77 amino acid residues.

序列

SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE

外觀

Lyophilized with no additives.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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R Hromas et al.
Biochemical and biophysical research communications, 255(3), 703-706 (1999-03-02)
Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue and play important roles in many disease processes. Chemokines are divided into two major groups, CC or CXC, based on their sequence around the amino terminal

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