SRP0135

G9a human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 同義詞: EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥70% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 74.6 kDa
• 包裝: pkg of 50 μg
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_006709
• UniProt登錄號: Q96KQ7
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... EHMT2(10919)

描述

一般說明

Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化／生理作用

The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3. Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis. G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor. It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy. G9a serves as a target for cancer therapy including breast cancer.

單位定義

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable