SAB5900203

Alpaca Anti-hTNFR, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• 同義詞: CD120a, TNFR1, tumor necrosis factor receptor superfamily member 1A, tumor necrosis factor receptor type 1
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: alpaca
• 品質等級: 100
• 重組細胞: expressed in E. coli
• 共軛: unconjugated
• 抗體表格: affinity purified VHH antibody
• 無性繁殖: TR09, monoclonal
• 化驗: >95% (SDS-PAGE)
• 形狀: lyophilized solid
• 分子量: 14.2 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• 純化經由: immobilized metal affinity chromatography (IMAC)
• 物種活性: human
• 濃度: 1 mg/mL
• 技術: ELISA: 1:1000-1:10000; immunofluorescence: 1:100-1:300; immunohistochemistry: 1:100-1:300; western blot: 1:500-1:3000
• 顏色: colorless
• pH值: ( in PBS, pH 7.4)
• 同型: VHH
• Protein ID登錄號: NP_001056.1
• UniProt登錄號: P19438
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TNFRSF1A(7132)

描述

一般說明

Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a (cluste of differentiation 120a), is an ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNFα). It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). TNFR1 can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.Alpaca derived anti-TNFR1 VHH single domain antibody (molecular weight: 14.2 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

特異性

Human Tumor necrosis factor receptor 1 (TNFR1) / CD120a

免疫原

Recombinant human TNFR1 (Ile22-Thr211) protein

應用

ELISA, WB, IHC, IF

包裝

50 µg per vial / 100 µg per vial

外觀

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.6

重構

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

儲存和穩定性

Stable for 1 year at 4°C from date of receipt.

其他說明

Storage at 4°C; Do not freeze.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable