SAB4200753

Anti-phospho-AKT (pThr⁴⁵⁰) antibody, Mouse monoclonal

clone AK-11, purified from hybridoma cell culture

• 同義詞: Anti-Protein Kinase B, Anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1
• 分類程式碼代碼: 12352203
• NACRES: NA.44

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: AK-11, monoclonal
• 形狀: buffered aqueous solution
• 分子量: ~56 kDa
• 物種活性: mouse, chicken, rat, human
• 濃度: ~1.0 mg/mL
• 技術: immunoblotting: 1-2 μg/mL using human breast cancer cell line MCF-7 extract; immunofluorescence: 1-2 μg/mL using mouse embryo fibroblast NIH-3T3 cells; immunoprecipitation (IP): 2-4 μg/test using whole extract of mouse embryo fibroblast NIH-3T3 cell line
• 同型: IgG2a
• UniProt登錄號: P31749
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pThr450)
• 基因資訊: human ... AKT1(207)

描述

一般說明

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal (mouse IgG2a isotype) is derived from the AK-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Serine/threonine kinase (AKT) also known as protein kinase B (PKB) belongs to the family of serine/threonine kinases. It consists of three isoforms such as AKT1, AKT2 and AKT3. AKT1 gene is located on the human chromosome at 14q32.33.

特異性

Anti-phospho-AKT (pThr450) antibody, Mouse monoclonal specifically recognizes all three AKT isoforms phosphorylated at Thr450 (AKT1, AKT2 and AKT3).

免疫原

Synthetic peptide corresponding to human AKT1 pThr450

應用

Anti-phospho-AKT (pThr450) antibody, mouse monoclonal may be used in:

• immunoblotting
• immunofluorescence
• immunoprecipitation

生化／生理作用

Serine/threonine kinase (AKT) plays an important role in the control of cell cycle and cell proliferation. It is also involved in cell differentiation and apoptosis signaling pathways. AKT is rapidly activated in response to cell stimulation by several growth factors, such as insulin, peroxyvanadate or cellular stresses (such as heat shock). Following activation, the phosphorylated AKT is relocated to the nucleus. Lack of phosphorylation at Thr450 enhances AKT ubiquitination and degradation.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable