SAB4200598

Monoclonal Anti-FTH1 antibody produced in mouse

~1.0 mg/mL, clone FTH24, purified immunoglobulin

• 同義詞: Monoclonal Anti-FHC, Monoclonal Anti-FTH, Monoclonal Anti-FTH1, Monoclonal Anti-FTHL6, Monoclonal Anti-OK/SW-cl.84, Monoclonal Anti-PIG15, Monoclonal Anti-PLIF, Monoclonal Anti-cell proliferation-inducing gene 15 protein, Monoclonal Anti-ferritin H subunit, Monoclonal Anti-ferritin heavy chain, Monoclonal Anti-ferritin, heavy polypeptide 1, Monoclonal Anti-placenta immunoregulatory factor, Monoclonal Anti-proliferation-inducing protein 15
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: FTH24, monoclonal
• 形狀: buffered aqueous solution
• 物種活性: human
• 濃度: ~1.0 mg/mL
• 技術: flow cytometry: 10-20 μg/test using HeLa cells; immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa).; indirect immunofluorescence: 5-10 μg/mL using HeLa cells
• 同型: IgG1
• UniProt登錄號: P02794
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FTH1(2495)

描述

一般說明

Monoclonal Anti-FTH1 (mouse IgG1 isotype) is derived from the hybridoma FTH24 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FTH1. Ferritin, a highly conserved iron-binding protein, is composed of two subunits, namely a 21 kDa heavy (H) chain and a 19 kDa light (L) chain. Ferritin heavy chain 1 gene is mapped on human chromosome 11q13. It is mainly found in heart and pancreas.

特異性

Monoclonal Anti- FTH1 recognizes human FTH1.

免疫原

synthetic peptide corresponding to an internal sequence of human FTH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

應用

Monoclonal Anti-FTH1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• flow cytometry
• immunofluorescence

生化／生理作用

Ferritin heavy chain catalyzes the first step in iron storage, the oxidation of Fe(II) to Fe(III). In addition to iron buffering, heavy chain ferritin also enhances de novo thymidine biosynthesis. Serum ferritin level is the most commonly used marker for determining iron status stored in the body. It is markedly influenced by factors such as inflammation, malnutrition, malignancy, and iron overload disorders. Studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases. FTH1 can also be used as a possible prognostic marker for breast cancer.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable