生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~90 kDa
物種活性
human, mouse
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
濃度
~1.5 mg/mL
技術
immunoprecipitation (IP): 10-20 μg using HEK-293T cell lysates overexpressing mouse BRSK1.
indirect immunofluorescence: 0.2-0.4 μg/mL using HEK-293T cells overexpressing mouse BRSK1.
western blot: 1-2 μg/mL using mouse forebrain (S1 fraction).
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... BRSK1(84446)
mouse ... Brsk1(381979)
一般說明
BRSK1 is a serine/threonine kinase that facilitates the G(2)/M cell cycle arrest upon UV- or methyl methane sulfonate-induced DNA damage. This kinase also modulates the polarity of neurons and duplication of centrosomes . Anti-BRSK1 (C-terminal) antibody is specific for mouse and human BRSK1. In immunoblotting, detection of the BRSK1 band is specifically inhibited by the BRSK1 immunizing peptide.
免疫原
synthetic peptide corresponding to a sequence at the C-terminal of human BRSK1, conjugated to KLH. The corresponding sequence is identical in mouse BRSK1.
應用
Anti-BRSK1 (C-terminal) antibody is suitable for use in western blot (1-2 μg/mL using S1 fraction extracts of mouse forebrain). The product can also be used for immunoprecipitation (10-20 μg) and indirect immunofluorescence (0.2-0.4 μg/mL) using HEK-293T cells overexpressing mouse BRSK1.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
María Alvarado-Kristensson et al.
Nature cell biology, 11(9), 1081-1092 (2009-08-04)
Symmetrical cell division requires duplication of DNA and protein content to generate two daughter cells. Centrosomes also duplicate during cell division, but the mechanism controlling this process is incompletely understood. We describe an alternative splice form of SadB encoding a
Rui Lu et al.
The Journal of biological chemistry, 279(30), 31164-31170 (2004-05-20)
Checkpoint activation by DNA damage during G(2) prevents activation of cyclin B/Cdc2 complexes, and as a consequence, mitotic entry is blocked. Although initiation and maintenance of G(2) arrest are known to be regulated by at least two distinct signaling pathways
Myriam Müller et al.
Journal of cell science, 123(Pt 2), 286-294 (2009-12-23)
Wee1 is well characterized as a cell-cycle checkpoint kinase that regulates the entry into mitosis in dividing cells. Here we identify a novel function of Wee1 in postmitotic neurons during the establishment of distinct axonal and dendritic compartments, which is
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